Human Red Blood Cell Experiments
Multiple experiments are performed with Human red blood cells.
Watching as intact live red blood cells change from oxygenated to deoxygenated as the environmental O2 is consumed.
The spectrophotometric recording of the release of O2 from HbO2 in human red blood cells (RBC). 300 nm span scans, collected at a rate of 2/second for 220 seconds:
The figure on the left clarifies what was shown in the 3D image. The graph to the right shows the kinetics.
The cells started oxygenated, the downward sloping line is the absorbance at 500 nm as the oxygen decreases, and the flat line at the bottom tells us that all of the HbO2 has released its oxygen:
Next experiment: The process of the HbO2 releasing from the RBCs
as they lysed during a 10 second time period. The data transition from very good spectra of HbO2 in intact RBC (figure on left) to very good spectra of free HbO2 in a clear buffer solution ( figure on right):
Download this document, showing the results of 3 unprecedented experiments possible only with the OLIS CLARiTY 1000.
Yeast Reduction Experiment
A dense yeast suspension undergoing a kinetic oxidation reduction event.
Spectra acquired with the Olis Clarity 1000A, an Olis RSM 1000 rapid-scanning spectrophotometer fitted with the DSPC (DeSa Suspension Presentation Chamber)
Yeast reducing during a 20 second time frame:
First spectrum, hence subtrahend:
All data used from that 20 second time period:
See experiments performed with whole mammalian cells, chunks of tissue, nanoparticles, frozen samples, aggregating proteins, and whole fruit. Send us your light scattering sample today for free proof of concept.