Immunity to Light Scatter Caused by Turbid Samples
“Difference spectra, ((CORM-3 plus reduced) minus reduced), of S. Typhimurium whole cells suspended in PBS were recorded in two different dual wavelength spectrophotometers. In this work, we have characterized the direct effects of CORM-3 on S. Typhimurium and demonstrate for the first time how CO released from CORM-3 binds to the terminal oxidases of the bacterium in situ in growing bacterial cultures under physiological conditions utilizing a commercial integrating cavity absorption meter (ICAM).”
Interaction of the carbon monoxide-releasing molecule Ru(CO)3Cl(glycinate) (CORM-3) with Salmonella enterica serovar Typhimurium: in situ measurements of CO binding by integrating cavity dual beam spectrophotometry.
Microbiology Papers in Press. 8.1.2014
Namrata Rana, Samantha McLean1, Brian E. Mann and Robert K. Poole
University of Sheffield
"We have developed a rapid (i.e., less than 20 minutes from culture to data), virtually real-time protocol to quantitate whole-cell Hb levels in turbid suspension. Briefly, high resolution UV/Vis absorbance scans from an Olis-CLARiTY RSM-ICAM that bracket the 413-nm Soret band for hemoglobin are combined with cell numbers from an automated, impedance-based counter to generate Hb concentration values.”
Results from a Cary 1G and CLARiTY 1000
Rapid Whole-Cell Hemoglobin Quantitation
Poster, 2014 Gordon Research Conference on Chemistry & Biology of Tetrapyrroles
Jason Marcero, Kyla Mohler, Joseph Burch, and Harry A. Dailey
University of Georgia
"We are accumulating data on several fronts using the CLARiTY. Here is a quick slide I did for a group meeting, showing what the CLARiTY can do for dilute phytoplankton suspensions (in this case, a culture of Prochlorococcus)."
Data in the graph compares the correct spectrum collected by the OLIS CLARiTY (blue) to the nondescript spectrum collected by a Shimadzu UV-1800 (red).
Doug Campbell, Mount Allison University
There is indeed a stark difference between our Perkin Elmer spectrophotometer and the CLARiTY instrument as you will notice on the attached spectra …They were recorded in the same conditions as yours…So we are more than ever willing to have one instrument bought."
Dan Lerner, University of Montpellier
A slide from an oral presentation at the 2014 International Food Technoloogy meeting comparing the CLARiTY's accurate spectra of whole milk and apple juice compared against her Cary 3C with and without integrated sphere.
Tatiana Koutchma, Agri-Food Canada
See the Performance Comparisons below!